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Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

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Abstract

Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. Structured illumination can be obtained by either laser interference or projection of fringe patterns. Here, we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device (DMD) which can be directly attached to most commercial inverted fluorescent microscopes and update it into a SIM system. The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured light field were studied. With the optimized fringe pattern, 1:6× resolution improvement could be obtained with high-end oil objectives. Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated. Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in the field of life science and medicine.

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基金项目:The study was funded by the National Key Technologies R&D Program of China (2018YFC0114800 and 2017YFC0109900), the Natural Science Foundation of China (NSFC) (61405238), the Natural Science Foundation of Jiangsu Province (BK20141206), and the Key Technologies R&D Program of Jiangsu Province (BE2018666).

收稿日期:2019-02-02

修改稿日期:2019-04-06

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Xibin Yang:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. ChinaUniversity of Chinese Academy of Sciences, Beijing 100049, P. R. China
Qian Zhu:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Zhenglong Sun:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Gang Wen:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Xin Jin:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Linbo Wang:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Jialin Liu:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Daxi Xiong:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China
Hui Li:Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, No. 88 Keling Street, Suzhou 215163, P. R. China

联系人作者:Daxi Xiong(xiongdx@sibet.ac.cn)

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引用该论文

Xibin Yang,Qian Zhu,Zhenglong Sun,Gang Wen,Xin Jin,Linbo Wang,Jialin Liu,Daxi Xiong,Hui Li. Fringe optimization for structured illumination super-resolution microscope with digital micromirror device[J]. Journal of Innovative Optical Health Sciences, 2019, 12(3): 1950014

Xibin Yang,Qian Zhu,Zhenglong Sun,Gang Wen,Xin Jin,Linbo Wang,Jialin Liu,Daxi Xiong,Hui Li. Fringe optimization for structured illumination super-resolution microscope with digital micromirror device[J]. Journal of Innovative Optical Health Sciences, 2019, 12(3): 1950014

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